Can green tea react with either DNA or calcium chloride?
I did an experiment to see if green tea can inhibit transformation. From long story to short, the green tea did prevent bacterial transformation from occurring in E. coli by disallowing the plasmid to enter through cell membrane. But my question is this: Was it possible for the green tea to react with the plasmid to break down the DNA or react with calcium chloride( which I used to make pores in the cell membrane and to neutralize the negatively charged DNA and the negatively charged cell membrane) so that it will prevent transformation? If it didn't, I could perhaps conclude that the green tea reacted with the cell membrane to force the pores to shut itself so that the DNA can't enter. But I'm not sure. Can you help me answer my question?
Kiyokuni YamadaQuestion asked by: kiyo777
Asked on: 30 Mar 2010
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Wow, that sounds like an interesting little experiment that you performed there!
I think the best way to get an answer to this would be to write it to a dedicated science site or perhaps to a university or if you are at school simply discuss it with the teacher of that subject.
Replied at: 05 Apr 2010Rate Answer
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