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Dilutions

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Dilutions

You have a bacteria culture with a concentration of 10 to the 6 cells per mL. Which dilution would you employ to dilute the culture so that if you plate 0.1 mL of the last dilution you will count 250 colonies on the plate?
Question asked by: renataassi

Asked on: 15 Mar 2010

So if you have 10^6 per ml then that is 1,000,000 bacteria. So in 0.1ml you would have 100,000 of them and you want just 250. That is 1/400th of what you have at present so you need to dilute it 400 times.
By: daveyc
Replied at: 16 Mar 2010
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